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Research Article Free access | 10.1172/JCI113439

Vascular transport of insulin to rat cardiac muscle. Central role of the capillary endothelium.

R S Bar, M Boes, and A Sandra

Department of Internal Medicine, Veterans Administration Medical Center, Iowa City, Iowa 52240.

Find articles by Bar, R. in: PubMed | Google Scholar

Department of Internal Medicine, Veterans Administration Medical Center, Iowa City, Iowa 52240.

Find articles by Boes, M. in: PubMed | Google Scholar

Department of Internal Medicine, Veterans Administration Medical Center, Iowa City, Iowa 52240.

Find articles by Sandra, A. in: PubMed | Google Scholar

Published April 1, 1988 - More info

Published in Volume 81, Issue 4 on April 1, 1988
J Clin Invest. 1988;81(4):1225–1233. https://doi.org/10.1172/JCI113439.
© 1988 The American Society for Clinical Investigation
Published April 1, 1988 - Version history
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Abstract

Using intact, beating hearts, we have assessed the interaction of insulin with capillary endothelium and the subsequent appearance of insulin in cardiac muscle. Rat hearts were perfused with 125I-insulin (10(-10) M) alone or in combination with unlabeled insulin (10(-9)-10(-5) M). 125I grains (shown to represent greater than 90% intact insulin) over both capillary endothelium and cardiac muscle decreased in a dose-dependent manner when hearts were co-perfused with labeled insulin and increasing concentrations of unlabeled insulin. Perfusion of 125I-desoctapeptide (DOP) insulin, a low affinity insulin analogue, with unlabeled insulin (10(-9)-10(-5) M) had no effect on the appearance of 125I-DOP insulin over microvessel endothelium and muscle. When capillary receptors were first destroyed by trypsin treatment or blocked by anti-receptor antibodies, the appearance of 125I-insulin in cardiac muscle decreased proportional to the inhibition of insulin binding to the capillary receptors. We conclude that insulin binding to capillary endothelial receptors is a central step in the transport of intravascular insulin to rat cardiac muscle.

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