Vascular biology
Abstract
Research Article
Authors
G. Matthew Longo, Wanfen Xiong, Timothy C. Greiner, Yong Zhao, Nicola Fiotti, B. Timothy Baxter
Abstract
Research Article
Authors
Lingyun Wu, Kun Cao, Yanjie Lu, Rui Wang
Abstract
Research Article
Authors
Christopher Heeschen, Michael Weis, Alexandra Aicher, Stefanie Dimmeler, John P. Cooke
Abstract
Research Article
Authors
Charles D. Loftin, Darshini B. Trivedi, Robert Langenbach
Abstract
Research Article
Authors
Masanori Ozaki, Seinosuke Kawashima, Tomoya Yamashita, Tetsuaki Hirase, Masayuki Namiki, Nobutaka Inoue, Ken-ichi Hirata, Hiroyuki Yasui, Hiromu Sakurai, Yuichi Yoshida, Masahiro Masada, Mitsuhiro Yokoyama
Abstract
Corrigendum
Authors
Qi Zhonghua, Chuan-Ming Hao, Robert I. Langenbach, Richard M. Breyer, Reyadh Redha, Jason D. Morrow, Matthew D. Breyer
Abstract
Research Article
Authors
Motoko Yanagita, Yoshikazu Ishimoto, Hidenori Arai, Kojiro Nagai, Tsuyoshi Ito, Toru Nakano, David J. Salant, Atsushi Fukatsu, Toshio Doi, Toru Kita
Abstract
Research Article
Authors
Lianchun Wang, Jillian R. Brown, Ajit Varki, Jeffrey D. Esko
Abstract
Research Article
Authors
Zhonghua Qi, Chuan-Ming Hao, Robert I. Langenbach, Richard M. Breyer, Reyadh Redha, Jason D. Morrow, Matthew D. Breyer
Abstract
Renin is an aspartyl protease essential for the control of blood pressure and was long suspected to have cellular receptors. We report the expression cloning of the human renin receptor complementary DNA encoding a 350–amino acid protein with a single transmembrane domain and no homology with any known membrane protein. Transfected cells stably expressing the receptor showed renin- and prorenin-specific binding. The binding of renin induced a fourfold increase of the catalytic efficiency of angiotensinogen conversion to angiotensin I and induced an intracellular signal with phosphorylation of serine and tyrosine residues associated to an activation of MAP kinases ERK1 and ERK2. High levels of the receptor mRNA are detected in the heart, brain, placenta, and lower levels in the kidney and liver. By confocal microscopy the receptor is localized in the mesangium of glomeruli and in the subendothelium of coronary and kidney artery, associated to smooth muscle cells and colocalized with renin. The renin receptor is the first described for an aspartyl protease. This discovery emphasizes the role of the cell surface in angiotensin II generation and opens new perspectives on the tissue renin-angiotensin system and on renin effects independent of angiotensin II.
Authors
Genevieve Nguyen, Françoise Delarue, Céline Burcklé, Latifa Bouzhir, Thomas Giller, Jean-Daniel Sraer
Copyright © 2020 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)