Glucocorticoids elevate GTP cyclohydrolase I mRNA levels in vivo and in PC12 cells
L Serova, B Nankova, M Rivkin, R Kvetnansky… - Molecular brain …, 1997 - Elsevier
GTP cyclohydrolase I (GTPCH) is the rate-limiting enzyme in the formation of
tetrahydrobiopterin, the cofactor for catecholamine, indolamine and nitric oxide biosynthesis.
The effect of glucocorticoids on GTPCH gene expression was examined by direct infusion of
cortisol to rats and by incubation of PC12 cells with glucocorticoids. Northern blot analysis
revealed that infusion of cortisol for 1 or 7 days elevated levels of the 3.6 kb GTPCH mRNA
species in rat adrenal medulla, while the 1.2 kb mRNA species were only increased by 1 …
tetrahydrobiopterin, the cofactor for catecholamine, indolamine and nitric oxide biosynthesis.
The effect of glucocorticoids on GTPCH gene expression was examined by direct infusion of
cortisol to rats and by incubation of PC12 cells with glucocorticoids. Northern blot analysis
revealed that infusion of cortisol for 1 or 7 days elevated levels of the 3.6 kb GTPCH mRNA
species in rat adrenal medulla, while the 1.2 kb mRNA species were only increased by 1 …