Rapid detection of MYCN gene amplification in neuroblastomas using the polymerase chain reaction
DCG Crabbe, J Peters, RC Seeger - Diagnostic Molecular …, 1992 - journals.lww.com
We have used the polymerase chain reaction (PCR) to detect amplification of the MYCN
oncogene in neuroblastoma cell lines and to distinguish primary tumors with a single copy
from those with MYCN amplification using DNA extracted from frozen sections. Two primer
pairs were used to co-amplify a 428-bp fragment of the MYCN oncogene along with a 268-
bp fragment of the (3-globin gene (a single-copy reference standard). After 30 cycles of
PCR, the products were resolved by agarose gel electrophoresis. MYCN gene amplification …
oncogene in neuroblastoma cell lines and to distinguish primary tumors with a single copy
from those with MYCN amplification using DNA extracted from frozen sections. Two primer
pairs were used to co-amplify a 428-bp fragment of the MYCN oncogene along with a 268-
bp fragment of the (3-globin gene (a single-copy reference standard). After 30 cycles of
PCR, the products were resolved by agarose gel electrophoresis. MYCN gene amplification …