Heparin affinity: purification of a tumor-derived capillary endothelial cell growth factor
Y Shing, J Folkman, R Sullivan, C Butterfield, J Murray… - Science, 1984 - science.org
Y Shing, J Folkman, R Sullivan, C Butterfield, J Murray, M Klagsbrun
Science, 1984•science.orgA tumor-derived growth factor that stimulates the proliferation of capillary endothelial cells
has a very strong affinity for heparin. This heparin affinity makes it possible to purify the
growth factor to a single-band preparation in a rapid two-step procedure. The purified growth
factor is a cationic polypeptide, has a molecular weight of about 18,000, and stimulates
capillary endothelial cell proliferation at a concentration of about 1 nanogram per milliliter.
has a very strong affinity for heparin. This heparin affinity makes it possible to purify the
growth factor to a single-band preparation in a rapid two-step procedure. The purified growth
factor is a cationic polypeptide, has a molecular weight of about 18,000, and stimulates
capillary endothelial cell proliferation at a concentration of about 1 nanogram per milliliter.
A tumor-derived growth factor that stimulates the proliferation of capillary endothelial cells has a very strong affinity for heparin. This heparin affinity makes it possible to purify the growth factor to a single-band preparation in a rapid two-step procedure. The purified growth factor is a cationic polypeptide, has a molecular weight of about 18,000, and stimulates capillary endothelial cell proliferation at a concentration of about 1 nanogram per milliliter.
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