Mucociliary clearance after lung denervation and bronchial transection

JS Brody, G Klempfner, MM Staum… - Journal of Applied …, 1972 - journals.physiology.org
JS Brody, G Klempfner, MM Staum, D Vidyasagar, DE Kuhl, JA Waldhausen
Journal of Applied Physiology, 1972journals.physiology.org
METHODS Surgical jxepara tion. Ten adult mongrel dogs underwent radical “hilar stripping”
of the left lung, a procedure which simulates the effects of lung autotransplantation but
avoids transection and reanastomosis of the pulmonary artery and veins (22). After the dogs
were anesthetized with intravenous pentobarbital, the chest was opened and the left main-
stem bronchus was divided within l-2 cm cf the carina. The pulmonary arteries and veins to
the left lung were stripped bare and all other left hilar structures were ligated and divided …
METHODS
Surgical jxepara tion. Ten adult mongrel dogs underwent radical “hilar stripping” of the left lung, a procedure which simulates the effects of lung autotransplantation but avoids transection and reanastomosis of the pulmonary artery and veins (22). After the dogs were anesthetized with intravenous pentobarbital, the chest was opened and the left main-stem bronchus was divided within l-2 cm cf the carina. The pulmonary arteries and veins to the left lung were stripped bare and all other left hilar structures were ligated and divided. The bronchus was reanastomosed with everting chromic catgut sutures, the chest was closed, and the residual pneumothorax was evacuated. All dogs were given penicillin and streptomycin intramuscularly daily for 7-10 days after surgery. The effectiveness of denervation was demonstrated by absence of the Hcring-Breuer inflation reflex in the operated lung (23). Bronchial clearance studies. Microspheres of albumin in the size range of 20-50 p were labeled with ggrrlTc using standard methods (4). The labeled microspheres were washed in turn with absolute ethanol and ether and then dried at 50 C for 15 min. Less than 1 7% of the initial gg* nTc label leached from the particles in a period of 1 hr.
The dried particles wcrc packed into the 0.5-mm diameter tip of a metal tube which was 80 cm in length. After the particles had been packed into the tube, a polyethylene catheter with its end sealed and with two l-mm side holes near the tip was placed over the end cf the metal tube. A 1 O-ml syringe filled with air was attached to the opposite end of the metal tube so that the particles could be insufflated into the bronchi by depression of the syringe barrel. The quantity of particles deposited with this system was determined by insufflating the particles into a test tube and by comparing the number of counts per minute obtained with the known specific activity of ggmTc, and was found to average 0.4 mg.
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