[17] A SELEX primer
T Fitzwater, B Polisky - Methods in enzymology, 1996 - Elsevier
Publisher Summary The SELEX (Systematic Evolution of Ligands by EXponential
enrichment) combinatorial chemistry process is a drug and diagnostic discovery technology
that permits the isolation of oligonucleotide ligands from large (10 15 members) random-
sequence single-stranded oligonucleotide populations that bind molecular targets, with high
affinity and specificity. An initial DNA oligonucleotide population is generated synthetically
and can serve as a transcription template to generate RNA pools for the RNA SELEX …
enrichment) combinatorial chemistry process is a drug and diagnostic discovery technology
that permits the isolation of oligonucleotide ligands from large (10 15 members) random-
sequence single-stranded oligonucleotide populations that bind molecular targets, with high
affinity and specificity. An initial DNA oligonucleotide population is generated synthetically
and can serve as a transcription template to generate RNA pools for the RNA SELEX …