Isolation and culture of mouse primary pancreatic acinar cells
J Gout, RM Pommier, DF Vincent, B Kaniewski… - JoVE (Journal of …, 2013 - jove.com
This protocol permits rapid isolation (in less than 1 hr) of murine pancreatic acini, making it
possible to maintain them in culture for more than one week. More than 20 x 106 acinar cells
can be obtained from a single murine pancreas. This protocol offers the possibility to
independently process as many as 10 pancreases in parallel. Because it preserves acinar
architecture, this model is well suited for studying the physiology of the exocrine pancreas in
vitro in contrast to cell lines established from pancreatic tumors, which display many genetic …
possible to maintain them in culture for more than one week. More than 20 x 106 acinar cells
can be obtained from a single murine pancreas. This protocol offers the possibility to
independently process as many as 10 pancreases in parallel. Because it preserves acinar
architecture, this model is well suited for studying the physiology of the exocrine pancreas in
vitro in contrast to cell lines established from pancreatic tumors, which display many genetic …