Functional Association between CBP and HNF4 inTrans-activation
E Yoshida, S Aratani, H Itou, M Miyagishi… - Biochemical and …, 1997 - Elsevier
Biochemical and biophysical research communications, 1997•Elsevier
CBP functions as a key transcriptional coactivator for a variety of transcription factors. We
show here that the hepatocyte nuclear factor 4 (HNF4), a transcription factor in the nuclear
receptor superfamily with no defined ligand, is cloned by yeast two-hybrid system using CBP
as a bait. GST-pull down assay with nuclear extracts orin vitrotranslation products revealed
that CBP and HNF4 interact with each other at the middle portion (aa 119–375) of HNF4 and
two distinct regions (aa 271–451 and 1626–2259) of CBP, respectively, in the ligand …
show here that the hepatocyte nuclear factor 4 (HNF4), a transcription factor in the nuclear
receptor superfamily with no defined ligand, is cloned by yeast two-hybrid system using CBP
as a bait. GST-pull down assay with nuclear extracts orin vitrotranslation products revealed
that CBP and HNF4 interact with each other at the middle portion (aa 119–375) of HNF4 and
two distinct regions (aa 271–451 and 1626–2259) of CBP, respectively, in the ligand …
CBP functions as a key transcriptional coactivator for a variety of transcription factors. We show here that the hepatocyte nuclear factor 4 (HNF4), a transcription factor in the nuclear receptor superfamily with no defined ligand, is cloned by yeast two-hybrid system using CBP as a bait. GST-pull down assay with nuclear extracts orin vitrotranslation products revealed that CBP and HNF4 interact with each other at the middle portion (aa 119–375) of HNF4 and two distinct regions (aa 271–451 and 1626–2259) of CBP, respectively, in the ligand-independent manner. Cotransfection experiments indicated that CBP is capable of activating HNF4 site-mediated transcription. These results suggested a functional association between CBP and HNF4 intrans-activation.
Elsevier