Kinetic and equilibrium analysis of the myosin ATPase
M Enrique, EM Ostap - Methods in enzymology, 2009 - Elsevier
The myosin superfamily consists of more than 35 classes (each consisting of multiple
isoforms) that have diverse cellular activities. The reaction pathway of the actin‐activated
myosin ATPase appears to be conserved for all myosin isoforms, but the rate and
equilibrium constants that define the ATPase pathway vary significantly across the myosin
superfamily, resulting in kinetic differences that that allow myosins to carry out diverse
mechanical functions. Therefore, it is important to determine the lifetimes and relative …
isoforms) that have diverse cellular activities. The reaction pathway of the actin‐activated
myosin ATPase appears to be conserved for all myosin isoforms, but the rate and
equilibrium constants that define the ATPase pathway vary significantly across the myosin
superfamily, resulting in kinetic differences that that allow myosins to carry out diverse
mechanical functions. Therefore, it is important to determine the lifetimes and relative …