Loss of filamin C is catastrophic for heart function
Circulation, 2020•Am Heart Assoc
A and B, Representative Western blot (A) and quantitation (B) showing efficient loss of Flnc
in icKO mouse (inducible cardiomyocyte-specific knockout) hearts 2 weeks after tamoxifen
treatment (TAM)(40 mg/kg/d for 3 days). Flncf/f; Cre− littermates after TAM served as controls
(Ctrl). Three mice per group were included. C, Kaplan-Meier survival curves of Flncf/f and
Flncf/f; Cre+ mice injected with TAM or vehicle control (n= 16, 8, 26, and 9, respectively). D,
Microscopic cross-sectional views of hematoxylin & eosin (left; scale bar, 1 mm) and Masson …
in icKO mouse (inducible cardiomyocyte-specific knockout) hearts 2 weeks after tamoxifen
treatment (TAM)(40 mg/kg/d for 3 days). Flncf/f; Cre− littermates after TAM served as controls
(Ctrl). Three mice per group were included. C, Kaplan-Meier survival curves of Flncf/f and
Flncf/f; Cre+ mice injected with TAM or vehicle control (n= 16, 8, 26, and 9, respectively). D,
Microscopic cross-sectional views of hematoxylin & eosin (left; scale bar, 1 mm) and Masson …
A and B, Representative Western blot (A) and quantitation (B) showing efficient loss of Flnc in icKO mouse (inducible cardiomyocyte-specific knockout) hearts 2 weeks after tamoxifen treatment (TAM)(40 mg/kg/d for 3 days). Flncf/f; Cre− littermates after TAM served as controls (Ctrl). Three mice per group were included. C, Kaplan-Meier survival curves of Flncf/f and Flncf/f; Cre+ mice injected with TAM or vehicle control (n= 16, 8, 26, and 9, respectively). D, Microscopic cross-sectional views of hematoxylin & eosin (left; scale bar, 1 mm) and Masson trichrome (right; scale bar: 50 μm) stained hearts isolated from Flnc icKO or Ctrl mice, 2 weeks or 6 weeks after TAM (n= 3). E, Heart weight to body weight (HW/BW) ratio for icKO (n= 14) and Ctrl (n= 12) mice 2 weeks after TAM.(Continued)
Am Heart Assoc