Molecular cloning and functional expression analysis of a cDNA for human hepassocin, a liver-specific protein with hepatocyte mitogenic activity
H Hara, H Yoshimura, S Uchida, Y Toyoda… - … et Biophysica Acta (BBA …, 2001 - Elsevier
By means of differential cDNA expression cloning, we earlier isolated a novel rat cDNA and
its protein, named hepassocin, which is upregulated during liver regeneration. Using the rat
cDNA as a probe, we have now isolated human hepassocin cDNA encoding a protein of
312 amino acids, which has 81.4% and 83.8% identity, respectively, to rat hepassocin
before and after elimination of its signal peptide. Dot blot analysis revealed that hepassocin
mRNA was strongly expressed in adult liver, fairly strongly in fetal liver, and weakly in …
its protein, named hepassocin, which is upregulated during liver regeneration. Using the rat
cDNA as a probe, we have now isolated human hepassocin cDNA encoding a protein of
312 amino acids, which has 81.4% and 83.8% identity, respectively, to rat hepassocin
before and after elimination of its signal peptide. Dot blot analysis revealed that hepassocin
mRNA was strongly expressed in adult liver, fairly strongly in fetal liver, and weakly in …