Mouse fetal liver culture system to dissect target gene functions at the early and late stages of terminal erythropoiesis
Erythropoiesis involves a dynamic process that begins with committed erythroid burst
forming units (BFU-Es) followed by rapidly dividing erythroid colony forming units (CFU-Es).
After CFU-Es, cells are morphologically recognizable and generally termed terminal
erythroblasts. One of the challenges for the study of terminal erythropoiesis is the lack of
experimental approaches to dissect gene functions in a chronological manner. In this
protocol, we describe a unique strategy to determine gene functions in the early and late …
forming units (BFU-Es) followed by rapidly dividing erythroid colony forming units (CFU-Es).
After CFU-Es, cells are morphologically recognizable and generally termed terminal
erythroblasts. One of the challenges for the study of terminal erythropoiesis is the lack of
experimental approaches to dissect gene functions in a chronological manner. In this
protocol, we describe a unique strategy to determine gene functions in the early and late …