Comparison of standard PCR/cloning to single genome sequencing for analysis of HIV-1 populations
To compare standard PCR/cloning and single genome sequencing (SGS) in their ability to
reflect actual intra-patient polymorphism of HIV-1 populations, a total of 530 HIV-1 pro-pol
sequences obtained by both sequencing techniques from a set of 17 ART naïve patient
specimens was analyzed. For each specimen, 12 and 15 sequences, on average, were
characterized by the two techniques. Using phylogenetic analysis, tests for panmixia and
entropy, and Bland–Altman plots, no difference in population structure or genetic diversity …
reflect actual intra-patient polymorphism of HIV-1 populations, a total of 530 HIV-1 pro-pol
sequences obtained by both sequencing techniques from a set of 17 ART naïve patient
specimens was analyzed. For each specimen, 12 and 15 sequences, on average, were
characterized by the two techniques. Using phylogenetic analysis, tests for panmixia and
entropy, and Bland–Altman plots, no difference in population structure or genetic diversity …