Flow cytometric analysis of cytokine expression in short-term allergen-stimulated T cells mirrors the phenotype of proliferating T cells in long-term cultures
D Van Hemelen, JNGO Elberink, B Bohle… - Journal of …, 2011 - Elsevier
BACKGROUND: Allergen-specific TH cells play an important role in IgE-mediated disorders
as allergies. Since this TH cell-population only accounts for a small percentage of TH cells,
they are difficult to phenotype without prior selection or expansion. METHODS: Grass-pollen-
specific TH cell profiles were evaluated in 5 allergic and 4 non-allergic individuals using
three different approaches: CD154 expression on ex vivo grass-pollen-activated PBMCs (i);
CFSE-dilution in grass-pollen-restimulated PBMCs (ii) and T cell lines enriched for allergen …
as allergies. Since this TH cell-population only accounts for a small percentage of TH cells,
they are difficult to phenotype without prior selection or expansion. METHODS: Grass-pollen-
specific TH cell profiles were evaluated in 5 allergic and 4 non-allergic individuals using
three different approaches: CD154 expression on ex vivo grass-pollen-activated PBMCs (i);
CFSE-dilution in grass-pollen-restimulated PBMCs (ii) and T cell lines enriched for allergen …