[HTML][HTML] Mucosal Immunization of Cynomolgus Macaques with the VSVΔG/ZEBOVGP Vaccine Stimulates Strong Ebola GP-Specific Immune Responses
X Qiu, L Fernando, JB Alimonti, PL Melito, F Feldmann… - PloS one, 2009 - journals.plos.org
PloS one, 2009•journals.plos.org
Background Zaire ebolavirus (ZEBOV) produces a lethal viral hemorrhagic fever in humans
and non-human primates. Methodology/Principal Findings We demonstrate that the
VSVΔG/ZEBOVGP vaccine given 28 days pre-challenge either intranasally (IN), orally (OR),
or intramuscularly (IM) protects non-human primates against a lethal systemic challenge of
ZEBOV, and induces cellular and humoral immune responses. We demonstrated that
ZEBOVGP-specific T-cell and humoral responses induced in the IN and OR groups …
and non-human primates. Methodology/Principal Findings We demonstrate that the
VSVΔG/ZEBOVGP vaccine given 28 days pre-challenge either intranasally (IN), orally (OR),
or intramuscularly (IM) protects non-human primates against a lethal systemic challenge of
ZEBOV, and induces cellular and humoral immune responses. We demonstrated that
ZEBOVGP-specific T-cell and humoral responses induced in the IN and OR groups …
Background
Zaire ebolavirus (ZEBOV) produces a lethal viral hemorrhagic fever in humans and non-human primates.
Methodology/Principal Findings
We demonstrate that the VSVΔG/ZEBOVGP vaccine given 28 days pre-challenge either intranasally (IN), orally (OR), or intramuscularly (IM) protects non-human primates against a lethal systemic challenge of ZEBOV, and induces cellular and humoral immune responses. We demonstrated that ZEBOVGP-specific T-cell and humoral responses induced in the IN and OR groups, following an immunization and challenge, produced the most IFN-γ and IL-2 secreting cells, and long term memory responses.
Conclusions/Significance
We have shown conclusively that mucosal immunization can protect from systemic ZEBOV challenge and that mucosal delivery, particularly IN immunization, seems to be more potent than IM injection in the immune parameters we have tested. Mucosal immunization would be a huge benefit in any emergency mass vaccination campaign during a natural outbreak, or following intentional release, or for mucosal immunization of great apes in the wild.
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