IgE and IgG4 epitope mapping by microarray immunoassay reveals the diversity of immune response to the peanut allergen, Ara h 2
WG Shreffler, DA Lencer, L Bardina… - Journal of Allergy and …, 2005 - Elsevier
BACKGROUND: Detailed assessment of antibody responses to allergens reveals clinically
relevant information about both host response and antigen structure. Microarray technology
offers advantages of scale and parallel design over previous methods of epitope mapping.
OBJECTIVE: We designed a redundant peptide microarray for IgE and IgG4 epitope
mapping of the previously characterized peanut allergen, Ara h 2. METHODS: Six complete
sets of overlapping peptides were commercially synthesized and site-specifically bound to …
relevant information about both host response and antigen structure. Microarray technology
offers advantages of scale and parallel design over previous methods of epitope mapping.
OBJECTIVE: We designed a redundant peptide microarray for IgE and IgG4 epitope
mapping of the previously characterized peanut allergen, Ara h 2. METHODS: Six complete
sets of overlapping peptides were commercially synthesized and site-specifically bound to …