[HTML][HTML] CRISPR/Cas9 deletion of ORMDLs reveals complexity in sphingolipid metabolism
CD Green, C Weigel, C Oyeniran, BN James… - Journal of Lipid …, 2021 - ASBMB
The serine palmitoyltransferase (SPT) complex catalyzes the rate-limiting step in the de
novo biosynthesis of ceramides, the precursors of sphingolipids. The mammalian ORMDL
isoforms (ORMDL1-3) are negative regulators of SPT. However, the roles of individual
ORMDL isoforms are unclear. Using siRNA against individual ORMDLs, only single
siORMDL3 had modest effects on dihydroceramide and ceramide levels, whereas
downregulation of all three ORMDLs induced more pronounced increases. With the …
novo biosynthesis of ceramides, the precursors of sphingolipids. The mammalian ORMDL
isoforms (ORMDL1-3) are negative regulators of SPT. However, the roles of individual
ORMDL isoforms are unclear. Using siRNA against individual ORMDLs, only single
siORMDL3 had modest effects on dihydroceramide and ceramide levels, whereas
downregulation of all three ORMDLs induced more pronounced increases. With the …