Myoglobin function reassessed

JB Wittenberg, BA Wittenberg - Journal of experimental …, 2003 - journals.biologists.com
JB Wittenberg, BA Wittenberg
Journal of experimental biology, 2003journals.biologists.com
The heart and those striated muscles that contract for long periods, having available almost
limitless oxygen, operate in sustained steady states of low sarcoplasmic oxygen pressure
that resist change in response to changing muscle work or oxygen supply. Most of the
oxygen pressure drop from the erythrocyte to the mitochondrion occurs across the capillary
wall. Within the sarcoplasm, myoglobin, a mobile carrier of oxygen, is developed in
response to mitochondrial demand and augments the flow of oxygen to the mitochondria …
Summary
The heart and those striated muscles that contract for long periods, having available almost limitless oxygen, operate in sustained steady states of low sarcoplasmic oxygen pressure that resist change in response to changing muscle work or oxygen supply. Most of the oxygen pressure drop from the erythrocyte to the mitochondrion occurs across the capillary wall. Within the sarcoplasm,myoglobin, a mobile carrier of oxygen, is developed in response to mitochondrial demand and augments the flow of oxygen to the mitochondria. Myoglobin-facilitated oxygen diffusion, perhaps by virtue of reduction of dimensionality of diffusion from three dimensions towards two dimensions in the narrow spaces available between mitochondria, is rapid relative to other parameters of cell respiration. Consequently, intracellular gradients of oxygen pressure are shallow, and sarcoplasmic oxygen pressure is nearly the same everywhere. Sarcoplasmic oxygen pressure, buffered near 0.33 kPa (2.5 torr; equivalent to approximately 4 μmol l-1 oxygen) by equilibrium with myoglobin, falls close to the operational Km of cytochrome oxidase for oxygen, and any small increment in sarcoplasmic oxygen pressure will be countered by increased oxygen utilization. The concentration of nitric oxide within the myocyte results from a balance of endogenous synthesis and removal by oxymyoglobin-catalyzed dioxygenation to the innocuous nitrate. Oxymyoglobin,by controlling sarcoplasmic nitric oxide concentration, helps assure the steady state in which inflow of oxygen into the myocyte equals the rate of oxygen consumption.
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