Long-term persistence of innate lymphoid cells in the gut after intestinal transplantation
Transplantation, 2017•journals.lww.com
Background Little is known about innate lymphoid cell (ILC) populations in the human gut,
and the turnover of these cells and their subsets after transplantation has not been
described. Methods Intestinal samples were taken from 4 isolated intestine and 3
multivisceral transplant recipients at the time of any operative resection, such as stoma
closure or revision. ILCs were isolated and analyzed by flow cytometry. The target
population was defined as being negative for lineage markers and double-positive for …
and the turnover of these cells and their subsets after transplantation has not been
described. Methods Intestinal samples were taken from 4 isolated intestine and 3
multivisceral transplant recipients at the time of any operative resection, such as stoma
closure or revision. ILCs were isolated and analyzed by flow cytometry. The target
population was defined as being negative for lineage markers and double-positive for …
Background
Little is known about innate lymphoid cell (ILC) populations in the human gut, and the turnover of these cells and their subsets after transplantation has not been described.
Methods
Intestinal samples were taken from 4 isolated intestine and 3 multivisceral transplant recipients at the time of any operative resection, such as stoma closure or revision. ILCs were isolated and analyzed by flow cytometry. The target population was defined as being negative for lineage markers and double-positive for CD45/CD127. Cells were further stained to define ILC subsets and a donor-specific or recipient-specific HLA marker to analyze chimerism.
Results
Donor-derived ILCs were found to persist greater than 8 years after transplantation. Additionally, the percentage of cells thought to be lymphoid tissue inducer cells among donor ILCs was far higher than that among recipient ILCs.
Conclusions
Our findings demonstrate that donor-derived ILCs persist long-term after transplantation and support the notion that human lymphoid tissue inducer cells may form in the fetus and persist throughout life, as hypothesized in rodents. Correlation between chimerism and rejection, graft failure, and patient survival requires further study.
Lippincott Williams & Wilkins