Acute testosterone deficiency alters adipose tissue fatty acid storage
S Santosa, NC Bush, MD Jensen - The Journal of Clinical …, 2017 - academic.oup.com
S Santosa, NC Bush, MD Jensen
The Journal of Clinical Endocrinology & Metabolism, 2017•academic.oup.comContext Although the long-term effects of testosterone on adipose tissue lipid metabolism in
men have been defined, the short-term regulation of these effects is not well understood.
Objective We examined the effects of acute testosterone withdrawal on subcutaneous
abdominal and femoral adipose tissue fatty acid (FA) storage and cellular mechanisms.
Design This was a prospective, randomized trial. Setting Mayo Clinic Clinical Research Unit.
Patients or Participants Thirty-two male volunteers ages 18 to 50 participated in these …
men have been defined, the short-term regulation of these effects is not well understood.
Objective We examined the effects of acute testosterone withdrawal on subcutaneous
abdominal and femoral adipose tissue fatty acid (FA) storage and cellular mechanisms.
Design This was a prospective, randomized trial. Setting Mayo Clinic Clinical Research Unit.
Patients or Participants Thirty-two male volunteers ages 18 to 50 participated in these …
Context
Although the long-term effects of testosterone on adipose tissue lipid metabolism in men have been defined, the short-term regulation of these effects is not well understood.
Objective
We examined the effects of acute testosterone withdrawal on subcutaneous abdominal and femoral adipose tissue fatty acid (FA) storage and cellular mechanisms.
Design
This was a prospective, randomized trial.
Setting
Mayo Clinic Clinical Research Unit.
Patients or Participants
Thirty-two male volunteers ages 18 to 50 participated in these studies.
Interventions
Volunteers were randomized to receive (1) no treatment (control), (2) injections (7.5 mg) of Lupron®, or (3) Lupron and testosterone (L+T) replacement for 49 days, resulting in 4 weeks of sex steroid suppression in the Lupron group.
Main Outcome Measures
We measured body composition, fat cell size, adipose tissue meal FA and direct free FA storage, lipoprotein lipase (LPL), acyl coenzyme A synthetase (ACS), diacylglycerol acyltransferase activities, and CD36 content.
Results
Compared with control and L+T groups, acute testosterone deficiency resulted in greater femoral adipose tissue meal FA storage rates, fasting and fed LPL activity, and ACS activity.
Conclusions
These results suggest that in men, testosterone plays a tonic role in restraining FA storage in femoral adipose tissue via suppression of LPL and ACS activities. FA storage mechanisms in men appear sensitive to short-term changes in testosterone concentrations.
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