[HTML][HTML] Humanized affinity-matured monoclonal antibody 8H9 has potent antitumor activity and binds to FG loop of tumor antigen B7-H3
M Ahmed, M Cheng, Q Zhao, Y Goldgur… - Journal of Biological …, 2015 - ASBMB
B7-H3 (CD276) is both an inhibitory ligand for natural killer cells and T cells and a tumor
antigen that is widely expressed among human solid tumors. Anti-B7-H3 mouse monoclonal
antibody 8H9 has been successfully used for radioimmunotherapy for patients with B7-H3
(+) tumors. We present the humanization, affinity maturation, and epitope mapping of 8H9
based on structure determination, modeling, and yeast display methods. The crystal
structure of ch8H9 Fab fragment was solved to 2.5-Å resolution and used as a template for …
antigen that is widely expressed among human solid tumors. Anti-B7-H3 mouse monoclonal
antibody 8H9 has been successfully used for radioimmunotherapy for patients with B7-H3
(+) tumors. We present the humanization, affinity maturation, and epitope mapping of 8H9
based on structure determination, modeling, and yeast display methods. The crystal
structure of ch8H9 Fab fragment was solved to 2.5-Å resolution and used as a template for …