RBL cells expressing human FcεRI are a sensitive tool for exploring functional IgE–allergen interactions: studies with sera from peanut-sensitive patients
DA Dibbern Jr, GW Palmer, PB Williams… - Journal of …, 2003 - Elsevier
Rat basophilic leukemia cells (RBL SX-38) express the α, β, and γ chains of human FcεRI.
Following sensitization with IgE from a subset of allergic human donors, these cells can be
triggered by exposure to anti-IgE or to very low concentrations of specific allergens. We
examined 18 sera from patients who were highly sensitive to peanuts by history and had anti-
peanut IgE by in vitro testing. The ability of these sera to sensitize the RBL SX-38 cells for
degranulation with peanut allergens correlates very well with the absolute amount of anti …
Following sensitization with IgE from a subset of allergic human donors, these cells can be
triggered by exposure to anti-IgE or to very low concentrations of specific allergens. We
examined 18 sera from patients who were highly sensitive to peanuts by history and had anti-
peanut IgE by in vitro testing. The ability of these sera to sensitize the RBL SX-38 cells for
degranulation with peanut allergens correlates very well with the absolute amount of anti …