The limitations and validities of senescence associated-β-galactosidase activity as an aging marker for human foreskin fibroblast Hs68 cells
NC Yang, ML Hu - Experimental gerontology, 2005 - Elsevier
The senescence associated-β-galactosidase (SA-βG) assay has become one of the most
commonly used markers of cell-aging. However, the reliability of the assay is questionable
because the enzyme is a non-specific marker for cell-aging. In this study, we found that the
SA-βG activity increased with cell age as well as in confluent quiescent cells or cells under
serum starvation, and in cells treated with H2O2. Importantly, we found that SA-βG activity
was irreversibly increased in the senescent cells or H2O2-teated cells, but was reversible in …
commonly used markers of cell-aging. However, the reliability of the assay is questionable
because the enzyme is a non-specific marker for cell-aging. In this study, we found that the
SA-βG activity increased with cell age as well as in confluent quiescent cells or cells under
serum starvation, and in cells treated with H2O2. Importantly, we found that SA-βG activity
was irreversibly increased in the senescent cells or H2O2-teated cells, but was reversible in …