[HTML][HTML] Optimized liquid and gas phase fractionation increases HLA-peptidome coverage for primary cell and tissue samples
MS is the most effective method to directly identify peptides presented on human leukocyte
antigen (HLA) molecules. However, current standard approaches often use 500 million or
more cells as input to achieve high coverage of the immunopeptidome, and therefore, these
methods are not compatible with the often limited amounts of tissue available from clinical
tumor samples. Here, we evaluated microscaled basic reversed-phase fractionation to
separate HLA peptide samples offline followed by ion mobility coupled to LC–MS/MS for …
antigen (HLA) molecules. However, current standard approaches often use 500 million or
more cells as input to achieve high coverage of the immunopeptidome, and therefore, these
methods are not compatible with the often limited amounts of tissue available from clinical
tumor samples. Here, we evaluated microscaled basic reversed-phase fractionation to
separate HLA peptide samples offline followed by ion mobility coupled to LC–MS/MS for …