Apolipoprotein B-100 destined for lipoprotein assembly and intracellular degradation undergoes efficient translocation across the endoplasmic reticulum membrane
MF Ingram, GS Shelness - Journal of lipid research, 1996 - ASBMB
It has been proposed that inefficient translocation across the endoplasmic reticulum (ER)
membrane gives rise to transmembrane forms of apolipoprotein B-100 (apoB). However, we
previously demonstrated that the amino-terminal 50% of apoB (apoB-50) was efficiently
translocated across the ER membrane in the nonhepatic cell line COS-1. To determine
whether liver-specific factors modulate apoB membrane translocation or topology, hybrid
proteins containing 300 amino acid overlapping segments of apoB-48 were transiently …
membrane gives rise to transmembrane forms of apolipoprotein B-100 (apoB). However, we
previously demonstrated that the amino-terminal 50% of apoB (apoB-50) was efficiently
translocated across the ER membrane in the nonhepatic cell line COS-1. To determine
whether liver-specific factors modulate apoB membrane translocation or topology, hybrid
proteins containing 300 amino acid overlapping segments of apoB-48 were transiently …