The basic understanding of inflammatory airway diseases greatly benefits from imaging the
cellular dynamics of immune cells. Current imaging approaches focus on labeling specific
cells to follow their dynamics but fail to visualize the surrounding tissue. To overcome this
problem, we evaluated autofluorescence multiphoton microscopy for following the motion
and interaction of cells in the airways in the context of tissue morphology. Freshly isolated
murine tracheae from healthy mice and mice with experimental allergic airway inflammation …