Fluorescence measurement of mitochondrial membrane potential changes in cultured cells
DG Nicholls - Mitochondrial Bioenergetics: Methods and Protocols, 2018 - Springer
Mitochondrial Bioenergetics: Methods and Protocols, 2018•Springer
The mitochondrial membrane potential is the dominant component of the proton-motive
force that is the potential term in the proton circuit linking electron transport to ATP synthesis
and other energy-dependent mitochondrial processes. Cationic fluorescent probes have
been used for many years to detect gross qualitative changes in mitochondrial membrane
potentials in intact cell culture. In this chapter I describe how these fluorescence signals may
be used to obtain a semiquantitative measure of changes in mitochondrial membrane …
force that is the potential term in the proton circuit linking electron transport to ATP synthesis
and other energy-dependent mitochondrial processes. Cationic fluorescent probes have
been used for many years to detect gross qualitative changes in mitochondrial membrane
potentials in intact cell culture. In this chapter I describe how these fluorescence signals may
be used to obtain a semiquantitative measure of changes in mitochondrial membrane …
Abstract
The mitochondrial membrane potential is the dominant component of the proton-motive force that is the potential term in the proton circuit linking electron transport to ATP synthesis and other energy-dependent mitochondrial processes. Cationic fluorescent probes have been used for many years to detect gross qualitative changes in mitochondrial membrane potentials in intact cell culture. In this chapter I describe how these fluorescence signals may be used to obtain a semiquantitative measure of changes in mitochondrial membrane potential.
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