[HTML][HTML] Targeted disruption of Np95 gene renders murine embryonic stem cells hypersensitive to DNA damaging agents and DNA replication blocks
M Muto, Y Kanari, E Kubo, T Takabe, T Kurihara… - Journal of Biological …, 2002 - ASBMB
NP95, which contains a ubiquitin-like domain, a cyclin A/E-Cdk2 phosphorylation site, a
retinoblastoma (Rb) binding motif, and a ring finger domain, has been shown to be
colocalized as foci with proliferating cell nuclear antigen in early and mid-S phase nuclei.
We established Np95 nulligous embryonic stem cells by replacing the exons 2–7 of theNp95
gene with a neo cassette and by selecting out a spontaneously occurring homologous
chromosome crossing over with a higher concentration of neomycin. Np95-null cells were …
retinoblastoma (Rb) binding motif, and a ring finger domain, has been shown to be
colocalized as foci with proliferating cell nuclear antigen in early and mid-S phase nuclei.
We established Np95 nulligous embryonic stem cells by replacing the exons 2–7 of theNp95
gene with a neo cassette and by selecting out a spontaneously occurring homologous
chromosome crossing over with a higher concentration of neomycin. Np95-null cells were …