PCR based determination of mitochondrial DNA copy number in multiple species
JP Rooney, IT Ryde, LH Sanders, EH Howlett… - … : Methods and Protocols, 2015 - Springer
Mitochondrial Regulation: Methods and Protocols, 2015•Springer
Mitochondrial DNA (mtDNA) copy number is a critical component of overall mitochondrial
health. In this chapter, we describe methods for isolation of both mtDNA and nuclear DNA
(nucDNA) and measurement of their respective copy numbers using quantitative PCR.
Methods differ depending on the species and cell type of the starting material and
availability of specific PCR reagents.
health. In this chapter, we describe methods for isolation of both mtDNA and nuclear DNA
(nucDNA) and measurement of their respective copy numbers using quantitative PCR.
Methods differ depending on the species and cell type of the starting material and
availability of specific PCR reagents.
Abstract
Mitochondrial DNA (mtDNA) copy number is a critical component of overall mitochondrial health. In this chapter, we describe methods for isolation of both mtDNA and nuclear DNA (nucDNA) and measurement of their respective copy numbers using quantitative PCR. Methods differ depending on the species and cell type of the starting material and availability of specific PCR reagents.
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