RNA polymerase II transcription in murine cells lacking the TATA binding protein
I Martianov, S Viville, I Davidson - Science, 2002 - science.org
I Martianov, S Viville, I Davidson
Science, 2002•science.orgInactivation of the murine TATA binding protein (TBP) gene by homologous recombination
leads to growth arrest and apoptosis at the embryonic blastocyst stage. However, after loss
of TBP, RNA polymerase II (pol II) remains in a transcriptionally active phosphorylation state,
and in situ run-on experiments showed high levels of pol II transcription comparable to those
of wild-type cells. In contrast, pol I and pol III transcription was arrested. Our results show a
differential dependency of the RNA polymerases on TBP and provide evidence for TBP …
leads to growth arrest and apoptosis at the embryonic blastocyst stage. However, after loss
of TBP, RNA polymerase II (pol II) remains in a transcriptionally active phosphorylation state,
and in situ run-on experiments showed high levels of pol II transcription comparable to those
of wild-type cells. In contrast, pol I and pol III transcription was arrested. Our results show a
differential dependency of the RNA polymerases on TBP and provide evidence for TBP …
Inactivation of the murine TATA binding protein (TBP) gene by homologous recombination leads to growth arrest and apoptosis at the embryonic blastocyst stage. However, after loss of TBP, RNA polymerase II (pol II) remains in a transcriptionally active phosphorylation state, and in situ run-on experiments showed high levels of pol II transcription comparable to those of wild-type cells. In contrast, pol I and pol III transcription was arrested. Our results show a differential dependency of the RNA polymerases on TBP and provide evidence for TBP-independent pol II transcriptional mechanisms that allow reinitiation and maintenance of gene transcription in vivo.
AAAS