Nucleolin and YB-1 are required for JNK-mediated interleukin-2 mRNA stabilization during T-cell activation
CY Chen, R Gherzi, JS Andersen… - Genes & …, 2000 - genesdev.cshlp.org
Genes & development, 2000•genesdev.cshlp.org
Regulated mRNA turnover is a highly important process, but its mechanism is poorly
understood. Using interleukin-2 (IL-2) mRNA as a model, we described a role for the JNK-
signaling pathway in stabilization of IL-2 mRNA during T-cell activation, acting via a JNK
response element (JRE) in the 5′ untranslated region (UTR). We have now identified two
major RNA-binding proteins, nucleolin and YB-1, that specifically bind to the JRE. Binding of
both proteins is required for IL-2 mRNA stabilization induced by T-cell activation signals and …
understood. Using interleukin-2 (IL-2) mRNA as a model, we described a role for the JNK-
signaling pathway in stabilization of IL-2 mRNA during T-cell activation, acting via a JNK
response element (JRE) in the 5′ untranslated region (UTR). We have now identified two
major RNA-binding proteins, nucleolin and YB-1, that specifically bind to the JRE. Binding of
both proteins is required for IL-2 mRNA stabilization induced by T-cell activation signals and …
Regulated mRNA turnover is a highly important process, but its mechanism is poorly understood. Using interleukin-2 (IL-2) mRNA as a model, we described a role for the JNK-signaling pathway in stabilization of IL-2 mRNA during T-cell activation, acting via a JNK response element (JRE) in the 5′ untranslated region (UTR). We have now identified two major RNA-binding proteins, nucleolin and YB-1, that specifically bind to the JRE. Binding of both proteins is required for IL-2 mRNA stabilization induced by T-cell activation signals and for JNK-induced stabilization in a cell-free system that duplicates essential features of regulated mRNA decay. Nucleolin and YB-1 are required for formation of an IL-2 mRNP complex that responds to specific mRNA stabilizing signals.
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