[HTML][HTML] Splicing factor SRp30c interaction with Y-box protein-1 confers nuclear YB-1 shuttling and alternative splice site selection
U Raffetseder, B Frye, T Rauen, K Jurchott… - Journal of Biological …, 2003 - ASBMB
The multifunctional DNA-and RNA-associated Y-box protein 1 (YB-1) specifically binds to
splicing recognition motifs and regulates alternative splice site selection. Here, we identify
the arginine/serine-rich SRp30c protein as an interacting protein of YB-1 by performing a
two-hybrid screen against a human mesangial cell cDNA library. Co-immunoprecipitation
studies confirm a direct interaction of tagged proteins YB-1 and SRp30c in the absence of
RNA via two independent protein domains of YB-1. A high affinity interaction is conferred …
splicing recognition motifs and regulates alternative splice site selection. Here, we identify
the arginine/serine-rich SRp30c protein as an interacting protein of YB-1 by performing a
two-hybrid screen against a human mesangial cell cDNA library. Co-immunoprecipitation
studies confirm a direct interaction of tagged proteins YB-1 and SRp30c in the absence of
RNA via two independent protein domains of YB-1. A high affinity interaction is conferred …