Autoantibodies, which are antibodies against self-antigens, are present in many disease
states and can serve as markers for disease activity. The levels of autoantibodies to specific
antigens are typically detected with the enzyme-linked immunosorbent assay (ELISA)
technique. However, screening for multiple autoantibodies with ELISA can be time-
consuming and requires a large quantity of patient sample. The antigen microarray
technique is an alternative method that can be used to screen for autoantibodies in a …