Induction of primary carcinoembryonic antigen (CEA)-specific cytotoxic T lymphocytes in vitro using human dendritic cells transfected with RNA

SK Nair, D Boczkowski, M Morse, RI Cumming… - Nature …, 1998 - nature.com
SK Nair, D Boczkowski, M Morse, RI Cumming, HK Lyerly, E Gilboa
Nature biotechnology, 1998nature.com
Dendritic cells (DC) generated from the peripheral blood mononuclear cells of healthy
individuals or from cancer patients transfected with carcinoembryonic antigen (CEA) mRNA
stimulate a potent CD8+ cytotoxic T lymphocyte (CTL) response in vitro. DCs are effectively
sensitized with RNA in the absence of reagents commonly used to facilitate mammalian cell
transfection. RNA encoding a chimeric CEA/LAMP-1 lysosomal targeting signal enhances
the induction of CEA-specific CD4+ T cells, providing a strategy to induce T-help that may be …
Abstract
Dendritic cells (DC) generated from the peripheral blood mononuclear cells of healthy individuals or from cancer patients transfected with carcinoembryonic antigen (CEA) mRNA stimulate a potent CD8+ cytotoxic T lymphocyte (CTL) response in vitro. DCs are effectively sensitized with RNA in the absence of reagents commonly used to facilitate mammalian cell transfection. RNA encoding a chimeric CEA/LAMP-1 lysosomal targeting signal enhances the induction of CEA-specific CD4+ T cells, providing a strategy to induce T-help that may be necessary to generate and/or maintain an optimal CD8+ CTL response in vivo. CEA RNA-transfected DCs also serve as effective targets in cytotoxicity assays, thus providing a general method for inducing, as well as measuring, CEA-specific CTL responses across a broad spectrum of HLA haplotypes.
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