Production of recombinant adeno‐associated viral vectors and use in in vitro and in vivo administration
SJ Gray, VW Choi, A Asokan… - Current protocols in …, 2011 - Wiley Online Library
Current protocols in neuroscience, 2011•Wiley Online Library
Adeno‐associated virus is a nonpathogenic human virus that has been developed into a
gene‐delivery vector due to its high efficiency of infection for many different cell types and its
ability to persist and lead to long‐term gene expression. This unit describes efficient
methods to generate high‐titer, research‐grade, adenovirus‐free recombinant single‐
stranded and self‐complementary adeno‐associated virus in various serotypes, along with
methods to quantify the viral vectors. Two detailed methods are provided for viral vector …
gene‐delivery vector due to its high efficiency of infection for many different cell types and its
ability to persist and lead to long‐term gene expression. This unit describes efficient
methods to generate high‐titer, research‐grade, adenovirus‐free recombinant single‐
stranded and self‐complementary adeno‐associated virus in various serotypes, along with
methods to quantify the viral vectors. Two detailed methods are provided for viral vector …
Abstract
Adeno‐associated virus is a nonpathogenic human virus that has been developed into a gene‐delivery vector due to its high efficiency of infection for many different cell types and its ability to persist and lead to long‐term gene expression. This unit describes efficient methods to generate high‐titer, research‐grade, adenovirus‐free recombinant single‐stranded and self‐complementary adeno‐associated virus in various serotypes, along with methods to quantify the viral vectors. Two detailed methods are provided for viral vector delivery into the rodent brain and spinal cord, and for histological detection of transgene expression of GFP. Curr. Protoc. Neurosci. 57:4.17.1‐4.17.30. © 2011 by John Wiley & Sons, Inc.
Wiley Online Library