[HTML][HTML] Discovery and characterization of a selective, nonpeptidyl thrombopoietin receptor agonist
CL Erickson-Miller, E DeLorme, SS Tian… - Experimental …, 2005 - Elsevier
OBJECTIVE: Peptide and other small molecule agonists have been described for several
cytokines and growth factors. Hydrazone compounds described here as thrombopoietin
receptor agonists were identified as activating STAT proteins in a Tpo responsive cell line.
METHODS: STAT activation and analysis of signal transduction pathways in cell lines and
normal human platelets was elucidated by Western blot and electrophoretic mobility shift
assays. Proliferation assays in cell types responsive to other cytokines determined specificity …
cytokines and growth factors. Hydrazone compounds described here as thrombopoietin
receptor agonists were identified as activating STAT proteins in a Tpo responsive cell line.
METHODS: STAT activation and analysis of signal transduction pathways in cell lines and
normal human platelets was elucidated by Western blot and electrophoretic mobility shift
assays. Proliferation assays in cell types responsive to other cytokines determined specificity …