[HTML][HTML] Identification of S6 kinase 1 as a novel mammalian target of rapamycin (mTOR)-phosphorylating kinase
Here we demonstrate that mammalian target of rapamycin (mTOR) is phosphorylated in a
rapamycin-sensitive manner. We show that S6 kinase 1 (S6K1), but not Akt, directly
phosphorylates mTOR in cell-free in vitro system and in cells. Expression of a constitutively
active, rapamycin-and wortmannin-resistant S6K1 leads to constitutive phosphorylation of
mTOR, whereas knock-down of S6K1 using small inhibitory RNA greatly reduces mTOR
phosphorylation despite elevated Akt activity. Importantly, phosphorylation of mTOR by …
rapamycin-sensitive manner. We show that S6 kinase 1 (S6K1), but not Akt, directly
phosphorylates mTOR in cell-free in vitro system and in cells. Expression of a constitutively
active, rapamycin-and wortmannin-resistant S6K1 leads to constitutive phosphorylation of
mTOR, whereas knock-down of S6K1 using small inhibitory RNA greatly reduces mTOR
phosphorylation despite elevated Akt activity. Importantly, phosphorylation of mTOR by …