A simple sensitive bioassay for interleukin-1 which is unresponsive to 103 U/ml of interleukin-2
AJH Gearing, CR Bird, A Bristow, S Poole… - Journal of immunological …, 1987 - Elsevier
Abstract A subclone, NOB-1, of the mouse EL-4 line constitutively produces very little
interleukin-2 but in response to interleukin-1 produces high concentrations of interleukin-2.
Co-stimulation with mitogen, phorbol esters or calcium ionophores was not required. NOB-1
is not responsive to tumour necrosis factor α, tumour necrosis factor β, interferon γ and
lipopolysaccharide. The NOB-1 line was used in conjunction with a CTLL line to detect less
than 1 pg/ml interleukin-1. Rapid assay was performed by co-culturing the EL-4 cells with …
interleukin-2 but in response to interleukin-1 produces high concentrations of interleukin-2.
Co-stimulation with mitogen, phorbol esters or calcium ionophores was not required. NOB-1
is not responsive to tumour necrosis factor α, tumour necrosis factor β, interferon γ and
lipopolysaccharide. The NOB-1 line was used in conjunction with a CTLL line to detect less
than 1 pg/ml interleukin-1. Rapid assay was performed by co-culturing the EL-4 cells with …