A technique to simultaneously visualize virus-specific CD8+ T cells and virus-infected cells in situ
Q Li, PJ Skinner, L Duan, AT Haase - JoVE (Journal of Visualized …, 2009 - jove.com
The numbers and locations of virus-specific CD8+ T cells relative to the numbers and
locations of their infected cell targets is thought to be critical in determining outcomes that
range from clearance to chronic persistent infections. We describe here a method for
assessing the spatial and quantitative relationships between immune effector (E) virus-
specific CD8+ T cells and infected targets (T) that combines in situ tetramer (IST) staining to
detect virus-specific CD8+ T cells and in situ hybridization (ISH) to detect viral-RNA+ cells in …
locations of their infected cell targets is thought to be critical in determining outcomes that
range from clearance to chronic persistent infections. We describe here a method for
assessing the spatial and quantitative relationships between immune effector (E) virus-
specific CD8+ T cells and infected targets (T) that combines in situ tetramer (IST) staining to
detect virus-specific CD8+ T cells and in situ hybridization (ISH) to detect viral-RNA+ cells in …