Tracking human antigen-specific memory B cells: a sensitive and generalized ELISPOT system
In the interest of better understanding the role of human memory B cells in protection against
disease, we developed an assay to quantitate antigen-specific memory B cells in human
blood. This assay utilizes a 6-day polyclonal stimulation of PBMC followed by an antigen-
specific ELISPOT for the detection of memory B cells that have differentiated into antibody
secreting cells (ASC) in vitro. We have used this assay to demonstrate that the anthrax
vaccine (AVA; BioThrax) elicits a substantial population of protective-antigen (PA) specific …
disease, we developed an assay to quantitate antigen-specific memory B cells in human
blood. This assay utilizes a 6-day polyclonal stimulation of PBMC followed by an antigen-
specific ELISPOT for the detection of memory B cells that have differentiated into antibody
secreting cells (ASC) in vitro. We have used this assay to demonstrate that the anthrax
vaccine (AVA; BioThrax) elicits a substantial population of protective-antigen (PA) specific …