Here we describe a method for detecting ultralow frequency target cells from within a high
background of irrelevant cells by a novel method, single epitope multiple staining (SEMS).
Samples of murine splenocytes were seeded with a low number of splenocytes from mice
transgenic for a hen eggwhite lysozyme (HEL)-specific immunoglobulin (Ig). These samples
were stained with two reagents specific for the same epitope expressed by the transgenic B
cells, which had been conjugated to two different detectable labels (FITC and biotin). This …