Localization of APOL1 protein and mRNA in the human kidney: nondiseased tissue, primary cells, and immortalized cell lines
L Ma, GS Shelness, JA Snipes, M Murea… - Journal of the …, 2015 - journals.lww.com
Although APOL1 gene variants are associated with nephropathy in African Americans, little
is known about APOL1 protein synthesis, uptake, and localization in kidney cells. To
address these questions, we examined APOL1 protein and mRNA localization in human
kidney and human kidney-derived cell lines. Indirect immunofluorescence microscopy
performed on nondiseased nephrectomy cryosections from persons with normal kidney
function revealed that APOL1 protein was markedly enriched in podocytes (colocalized with …
is known about APOL1 protein synthesis, uptake, and localization in kidney cells. To
address these questions, we examined APOL1 protein and mRNA localization in human
kidney and human kidney-derived cell lines. Indirect immunofluorescence microscopy
performed on nondiseased nephrectomy cryosections from persons with normal kidney
function revealed that APOL1 protein was markedly enriched in podocytes (colocalized with …