[HTML][HTML] Identification of 14-3-3ζ as a protein kinase B/Akt substrate
DW Powell, MJ Rane, Q Chen, S Singh… - Journal of Biological …, 2002 - ASBMB
Protein kinase B/Akt (PKB/Akt) is a member of the ACG kinase family, which also includes
protein kinase C, that phosphorylates a number of 14-3-3-binding proteins. 14-3-3 protein
regulation of protein kinase C activity is modulated by 14-3-3 phosphorylation. We examined
the hypothesis that PKB/Akt interacts with and phosphorylates 14-3-3ζ, leading to
modulation of dimerization. By glutathione S-transferase pull-down, Akt precipitated
recombinant 14-3-3ζ and endogenous 14-3-3ζ from HEK293 cell lysates. Recombinant …
protein kinase C, that phosphorylates a number of 14-3-3-binding proteins. 14-3-3 protein
regulation of protein kinase C activity is modulated by 14-3-3 phosphorylation. We examined
the hypothesis that PKB/Akt interacts with and phosphorylates 14-3-3ζ, leading to
modulation of dimerization. By glutathione S-transferase pull-down, Akt precipitated
recombinant 14-3-3ζ and endogenous 14-3-3ζ from HEK293 cell lysates. Recombinant …