[HTML][HTML] Identification of regulatory sites of phosphorylation of the bovine endothelial nitric-oxide synthase at serine 617 and serine 635
BJ Michell, MB Harris, Z Chen, H Ju, VJ Venema… - Journal of Biological …, 2002 - ASBMB
Endothelial nitric-oxide synthase (eNOS) is regulated by signaling pathways involving
multiple sites of phosphorylation. The coordinated phosphorylation of eNOS at Ser 1179 and
dephosphorylation at Thr 497 activates the enzyme, whereas inhibition results when Thr 497
is phosphorylated and Ser 1179 is dephosphorylated. We have identified two further
phosphorylation sites, at Ser 617 and Ser 635, by phosphopeptide mapping and matrix-
assisted laser desorption ionization time of flight mass spectrometry. Purified protein kinase …
multiple sites of phosphorylation. The coordinated phosphorylation of eNOS at Ser 1179 and
dephosphorylation at Thr 497 activates the enzyme, whereas inhibition results when Thr 497
is phosphorylated and Ser 1179 is dephosphorylated. We have identified two further
phosphorylation sites, at Ser 617 and Ser 635, by phosphopeptide mapping and matrix-
assisted laser desorption ionization time of flight mass spectrometry. Purified protein kinase …