Materials and Methods

Toxins and Fragments. Tetanus toxin, a kind gift ofDr. Labert (Instituí Pasteur Production, Louviers, France), was

1 Abbreviations: TeTx, tetanus toxin; L, light chain of tetanus toxin; H, heavy chain of tetanus toxin; DT, diphtheria toxin; SDS, sodium dodecyl sulfate; GDlb, galactosyl-jV-acetylgalactosaminyl-N-acetylneuraminyl-JV-acetylneuraminylgalactosylglucosylceramide; EL, egg lecithin; DML, dimyristoyllecithin; MA, myristic acid;[3H] PC I, 1-palmitoyl-2-(2-azido-4-nitrobenzoyl)-jn-glycero-3-[3H] phosphocholine;[14C] PC II, 1-myristoyl-2-[12-[(4-azido-2-nitrophenyl) amino] dodecanoyl]-rn-glycero-3-['4C] phosphocholine;[3H] PC III, 1-palmitoyl-2-[10-[4-[(trifluoromethyl) diazirinyl] phenyl]-8-oxa [9-3H] decanoyl]-in-glycero-3-phosphocholine; Tris, tris (hydroxymethyl) aminomethane.