[HTML][HTML] Determinants of microRNA processing inhibition by the developmentally regulated RNA-binding protein Lin28
E Piskounova, SR Viswanathan, M Janas… - Journal of Biological …, 2008 - ASBMB
The developmentally regulated RNA-binding protein Lin28 blocks processing of let-7 family
microRNAs (miRNAs) in embryonic cells. The molecular basis for this selective miRNA
processing block is unknown. Here we find that Lin28 selectively binds the terminal loop
region of let-7 precursors in vitro and that the loop mediates miRNA processing inhibition in
vivo. Additionally, we identify the domains of Lin28 required for this inhibition. These findings
establish a regulatory role for the terminal loop of precursors in miRNA maturation and …
microRNAs (miRNAs) in embryonic cells. The molecular basis for this selective miRNA
processing block is unknown. Here we find that Lin28 selectively binds the terminal loop
region of let-7 precursors in vitro and that the loop mediates miRNA processing inhibition in
vivo. Additionally, we identify the domains of Lin28 required for this inhibition. These findings
establish a regulatory role for the terminal loop of precursors in miRNA maturation and …
