CD4+ T-Cell Recognition of Mutated B-RAF in Melanoma Patients Harboring the V599E Mutation
MS Sharkey, G Lizée, MI Gonzales, S Patel… - Cancer research, 2004 - AACR
MS Sharkey, G Lizée, MI Gonzales, S Patel, SL Topalian
Cancer research, 2004•AACRThe potential of antigen-directed cancer immunotherapy has not been fully realized,
perhaps because many commonly targeted tumor associated proteins are not essential to
maintaining the malignant cell phenotype. A constitutively activating mutation in the
signaling molecule BRAF is expressed frequently in melanomas and may play an important
role in the biology of this disease. A 29-mer B-Raf peptide incorporating the V599E mutation
was used for in vitro stimulation of lymphocytes derived from melanoma patients, generating …
perhaps because many commonly targeted tumor associated proteins are not essential to
maintaining the malignant cell phenotype. A constitutively activating mutation in the
signaling molecule BRAF is expressed frequently in melanomas and may play an important
role in the biology of this disease. A 29-mer B-Raf peptide incorporating the V599E mutation
was used for in vitro stimulation of lymphocytes derived from melanoma patients, generating …
Abstract
The potential of antigen-directed cancer immunotherapy has not been fully realized, perhaps because many commonly targeted tumor associated proteins are not essential to maintaining the malignant cell phenotype. A constitutively activating mutation in the signaling molecule BRAF is expressed frequently in melanomas and may play an important role in the biology of this disease. A 29-mer B-Raf peptide incorporating the V599E mutation was used for in vitro stimulation of lymphocytes derived from melanoma patients, generating MHC class II-restricted CD4+ T cells specific for this peptide as well as for melanoma cells expressing B-Raf V599E. Mutated B-Raf exemplifies targets that may be ideal for immunotherapy.
AACR