Hypoxia limits differentiation and up-regulates expression and activity of prostaglandin H synthase 2 in cultured trophoblast from term human placenta
DM Nelson, RD Johnson, SD Smith, EY Anteby… - American journal of …, 1999 - Elsevier
Objective: We determined the effect of hypoxic conditions on cellular differentiation and
prostaglandin H synthase expression in cultured human term trophoblast. Study Design:
Cytotrophoblasts isolated from term placentas were cultured for 24-72 hours in a standard
(20% oxygen) or hypoxic (1% to 2% oxygen) atmosphere. Trophoblast biochemical
differentiation was determined by release of human chorionic gonadotropin. Morphologic
differentiation was evaluated by epifluorescent and confocal microscopic examination of …
prostaglandin H synthase expression in cultured human term trophoblast. Study Design:
Cytotrophoblasts isolated from term placentas were cultured for 24-72 hours in a standard
(20% oxygen) or hypoxic (1% to 2% oxygen) atmosphere. Trophoblast biochemical
differentiation was determined by release of human chorionic gonadotropin. Morphologic
differentiation was evaluated by epifluorescent and confocal microscopic examination of …