[HTML][HTML] High resolution clear native electrophoresis for in-gel functional assays and fluorescence studies of membrane protein complexes
I Wittig, M Karas, H Schagger - Molecular & Cellular Proteomics, 2007 - ASBMB
Clear native electrophoresis and blue native electrophoresis are microscale techniques for
the isolation of membrane protein complexes. The Coomassie Blue G-250 dye, used in blue
native electrophoresis, interferes with in-gel fluorescence detection and in-gel catalytic
activity assays. This problem can be overcome by omitting the dye in clear native
electrophoresis. However, clear native electrophoresis suffers from enhanced protein
aggregation and broadening of protein bands during electrophoresis and therefore has …
the isolation of membrane protein complexes. The Coomassie Blue G-250 dye, used in blue
native electrophoresis, interferes with in-gel fluorescence detection and in-gel catalytic
activity assays. This problem can be overcome by omitting the dye in clear native
electrophoresis. However, clear native electrophoresis suffers from enhanced protein
aggregation and broadening of protein bands during electrophoresis and therefore has …